The chemical proteomics technology service platform can be applied to different sample systems, including cell lysates, living cells, and animal or human tissues. It can solve post-translational modification of protein analysis, drug target identification and metabolite binding protein analysis, etc. Important biological issues.


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Technology platform one:

Schematic diagram of Gel-based activity-based protein profiling. The use of specific chemical probes can quickly analyze the activity of proteins or enzymes in the sample, and can also be used to detect the labeling ability of chemical probes and label targets. At the same time, its associated technology (competitive activity-based protein profiling) can also be used to quickly and efficiently discover small molecule inhibitors. (Activity-Based Protein Profiling: From Enzyme Chemistry to Proteomic Chemistry. Annu. Rev. Biochem. 2008, 77, 383-414.)








  Technology platform two:

  Schematic diagram of Liquid chromatography-mass spectrometry-based platforms for activity-based protein profiling. This technology platform can not only identify the protein labeled by the chemical probe, but also be accurate to the labeling site. Combined with quantitative proteomics technology, such as ReDiMe, SILAC, iTRAQ/TMT, it can simultaneously perform protein and site double quantitative analysis of the target protein labeled by the chemical probe. (Tandem orthogonal proteolysis-activity-based protein profiling (TOP-ABPP)--a general method for mapping sites of probe modification in proteomes. Nat Protoc. 2007, 2, 1414-25.)